Macroscopic and histopathological features of acute peritoneal infection 
in diabetic and euglycemic Wistar rats

Oleksandr Yakymenko; Svitlana Suchok; Oleg Lukiianets; Andrii Havryliuk

doi:10.5114/pedm.2022.112497

eISSN: 2083-8441
ISSN: 2081-237X

Pediatric Endocrinology Diabetes and Metabolism

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abstract:

Original paper

Macroscopic and histopathological features of acute peritoneal infection in diabetic and euglycemic Wistar rats

Oleksandr Yakymenko

¹

,

Svitlana Suchok

¹

,

Oleg Lukiianets

¹

,

Andrii Havryliuk

¹

Department of Pediatric Surgery, National Pirogov Memorial Medical University, Vinnytsya, Ukraine

Pediatr Endocrinol Diabetes Metab 2022; 28 (1): 30–34

DOI: https://doi.org/10.5114/pedm.2022.112497

Online publish date: 2022/01/26

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Introduction
Impact of the secondary peritoneal infections on the peritoneal morphology in diabetic population remains unclear.

Aim of the study
To study the histopathological changes of the peritoneum due to secondary bacterial peritonitis in diabetic rats in compari-son to the normoglycemic animals.

Material and methods
13 adult male Wistar rats were divided into 2 groups: control (n = 3) and experimental (n = 10). The latter was subdivided into non-diabetic (n = 5) and diabetic (n = 5). T1DM and peritonitis were induced through the single intraperitoneal injec-tion of Streptozotocin (60 mg/kg) and intraperitoneal injection of 2 ml of the faecal matter, respectively.

Results
Macroscopic findings of diabetic rat peritoneum included significant accumulation of the exudate in the peritoneal cavity and presence of intraintestinal adhesions and abscesses. Morphology of the peritonium presented with diffuse, profound degenerative and inflammatory lesions involving the subjacent tissues. In contrast, the underlying muscular layer of the non-diabetic rats remained intact.

Conclusions
Natural course of the peritoneal infection in diabetic rats (48 hours) is characterised by diffuse severe peritonitis with inter-intestinal abscess formation and more profound subadjacent tissue involvement in comparison with non-diabetic rats.

keywords:

Macroscopic and histopathological features of acute peritoneal infection in diabetic and euglycemic Wistar rats

Oleksandr Yakymenko 1 , Svitlana Suchok 1 , Oleg Lukiianets 1 , Andrii Havryliuk 1

peritoneum, diabetes mellitus, Wistar rat, peritonitis

Oleksandr Yakymenko

¹

,

Svitlana Suchok

¹

,

Oleg Lukiianets

¹

,

Andrii Havryliuk

¹