Immuno-physiological assessment for some biomarkers of patients with reproductive disorders

Aim:
The purpose of this study was to evaluate several physiological and immunological markers in individuals experiencing disorders in their reproductive system.

Material and methods:
C-reactive protein (CRP) and Rheumatoid Factor (RF). Additionally, the physiological indicators analyzed in this study included Age, Weight and Length, Vitamin D, Antimullerian hormone (AMH), Testosterone, Lipid profile (Cholesterol, Triglyceride, HDL, LDL, VLDL), Fasting blood sugar (FBS), Insulin, and Hemoglobin A1c (HbA1c). However, this study featured three distinct groups: Group – A (Normospermia), Group- B (Patients with Oligospermia), and Group- C (Patients with Azospermia). The Cobas e 411 analyzer was employed for the estimation of Vitamin D and Insulin. The measurement of AMH and Testosterone was carried out via the Sandwich Enzyme-Linked Immune-Sorbent assay (ELISA), while FBS and Lipid profiles were examined through the utilization of a spectrophotometer (Enzymatic colorimetric method). The concentrations of CRP, RF, and HbA1c were estimated using the Abbott Architect c4000 analyzer.

Results:
The difference exists (p<0.05) between the three groups about indicators such as age, weight, vitamin D, AMH, testosterone, cholesterol, and insulin. The study further divulges that, in contrast, no significant differences were detected among the three groups concerning other parameters, including length, CRP, RF, Triglyceride, HDL, LDL, VLDL, FBS, and HbA1c.

Conclusions:
The identification of abnormalities in the male reproductive system requires the integration of both immunological and physiological markers. An evident disparity in the levels of the aforementioned markers was noted in individuals suffering from oligospermia and azoospermia as compared to those with normospermia.